FAQ

THE ONLY BANKING RULE IS THE MYCORD POOL

How much cord blood is needed for a transplant?

The volume of the cord blood collected after the pregnancy determines the outcome of the transplantation. The average size of private cord blood collections is 60mL. This small volume corresponds to 470 million Total Nucleated cells or 1.8 million CD34 + stem cells which is not sufficient for a successful transplantation. In Mycord, we understand the volume of cord blood to be collected is 80mL and more. This 80mL of cord blood corresponds to more than 600 million Total Nucleated cells or over 2 million CD 34+ stem cells per unit.

Reference :  US FDA Guideline 2009     Transplantation and Cellular Engineering

What is the desired TNC and CD34+ stem cells per unit of cord blood stored?

According to US FDA guidelines 2009, cord blood unit should contain more than 500 Million Total nucleated cells per unit and viable CD34+ Hematopoietic stem cells should be more than 1.25 million cells per unit before cryopreservation.

Reference :  US FDA Guideline 2009

What is the actual requirement of TNC per Kg for successful transplantation?

According to US FDA guidelines 2009, the transplant dose should be at least 25 million TNC per kilogram body weight before cryopreservation. The post thawed sample should contain 17 million TNC per kilogram body weight.

Reference :  US FDA Guideline 2009

What should be the minimal volume of cord blood to be collected to achieve 25 million TNC per Kg for a successful transplantation?

The crucial thing is the volume of the cord blood collection. Mycord recommends minimum of 80mL. More the volume of the cord blood over 100mL collected more TNC and more CD 34+ stem cells.

Reference :  US FDA Guideline 2009

Does the viability affect the efficacy of cord blood unit retrieved during transplantation?

Yes. Viability of the cord blood is one of the crucial factors for a successful transplantation. According to US FDA guidelines 2009, 85 % viable nucleated cells per unit should be present before cryopreservation.

Reference :  US FDA Guideline 2009

What happens when a low volume of cord blood is stored?

Since Umbilical cord blood hematopoietic stem cells expansion is still in research stage, blood units with less volume should be of no use to the parents after storage. Awareness should be created by the government to educate the masses to avoid units with no adequate CD 34 and TNCC cells to be stored.

What is Dual storage, does it has an impact on the cord blood transplantation?

Dual storage is splitting the same cord blood units and storing it in two different locations. No it has no relevance for successful cord blood transplantation. Dual storage schemes introduced in the market by the current cord blood banking companies is a gimmick where in the adequate cells needed per unit for the transplantation is not achieved, thereby storing the same units on dual storage scheme is only a waste to the parents and adequate awareness should be given to the expected parents on the importance of volume, CD34+ cells, TNCC to be needed for a successful hematopoietic transplantation.

What certifications/accreditation’s does MyCord (CelluGen) possess?

CelluGen Cord Blood Bank is licensed by DCGI and ISO9001:2008 certified.

How does Mycord process cord blood units?

Mycord uses Manual Method of Cord Blood processing using the principle “Volume Reduction” – using Hydroxyl Ethyl Starch for Red Blood cells sedimentation and removal and Plasma depletion thereby concentrating on the White blood cells containing Hematopoietic progenitor cells.

What is the comparison of Manual cord blood processing with automated cord blood processing?

The results of the manual method of processing showed an average of TNC recovery of 83.4% compared to SEPAX with TNC recovery of 75%.

Reference :  CESCA Therapeutics – Meta Analysis Report

What is the disadvantage of automated method such as sepax over manual cord blood processing?

The CD 34+ Stem cells and its yield was not satisfactory in the cord blood units processed using sepax method, when retrieved and thawed compared to cord blood units processed manually. There is a relative lower efficiency of CD34+ stem cells cryopreservation in sepax methodology, since system contains only small bicompartmental bags resulting in improper homogenization of DMSO –dextran with the cell suspension.

Reference :  Manual-Cord-Blood-Processing

What is the advantage of Manual cord blood processing method over SEPAX oh Homogenization of Cell suspension with Cryoprotectant

In Manual Methodology, the larger volume of the processing bag permits better homogenization of DMSO-Dextran with the cell suspension before transferring the final suspension into small bags for cryopreservation.

Reference :  Manual Cord Blood Processing

How long cord blood can be stored?

No one knows for sure the shelf life of the cord blood. Published studies indicate that UCB stem cells cryopreserved for 21-23.5 years have manifested biologic qualities equal to those at the time they were frozen.

Reference :  Duration of cord blood storage AABB

What is the volume of final product of Cord blood which is cryopreserved?

The volume of the cord blood collected after the pregnancy determines the outcome of the transplantation. The average size of private cord blood collections is 60mL. This small volume corresponds to 470 million Total Nucleated cells or 1.8 million CD34 + stem cells which is not sufficient for a successful transplantation. In Mycord, we understand the volume of cord blood to be collected is 80mL and more. This 80mL of cord blood corresponds to more than 600 million Total Nucleated cells or over 2 million CD 34+ stem cells per unit.

What is the advantage of using Hydroxyl Ethyl Starch for Red Blood cell reduction and Plasma Depletion Method?

Average recovery percentage of Total Nucleated cells and CD34 % in UCB using Hydroxy Ethyl Starch is 94.1 % compared to Sepax is 92.3%.

Average recovery of Mononuclear cells % in UCB using HydroxyEthyl starch is 80.9 % compared to sepax 79.9%

Reference :  Cord Blood Processing

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